FIG 1.

Experimental scheme and infectious progeny production of HCV populations. (A) Passage history and origin of HCV populations (filled circles) and biological clones (filled squares) used in the present study. The initial HCV (gray square) was derived by transcription of plasmid Jc1FLAG2(p7-nsGluc2A). Virus passages are indicated by arrows; p denotes passage number; and (a), (b), and (c) indicate three replicate passages. (B) Extracellular infectious viral titer produced by the triplicate populations shown in the box above the panels. Procedures and infection conditions are detailed in Materials and Methods.