FIG 5.

Effect of CD4 and SERINC5 on Env conformation. 293T cells expressing or not CD4 were transfected with the indicated provirus alone or cotransfected with a plasmid expressing SERINC5-HA. (a) Infectivity of viral particles tested using the TZM-bl infectivity assay. The means and SEM of two independent experiments are shown. (b) Radar plot analyses of the Env profile in transfected cells (left) or viral aggregates (right) in the presence (red) or absence (black) of SERINC5. The percentages were calculated by flow cytometry or flow virometry, respectively. The means of two independent experiments are shown. Statistical analysis was performed using Prism software and a multiple unpaired t test. *, P < 0.05; **, P < 0.005; ***, P < 0.0005.