FIG 3.

Phylogenetic tree analysis of donor 236 vaccinated with Fluzone. (a) Circular phylogram tree drawn using 457 unique paired heavy and light chain antibody sequences showing a highly diverse plasmablast antibody repertoire at day 7. (b) Singleton MAbs from HV4 family preferably paired with different lambda light chains and were highly mutated, including C585 (shown with an arrow). (c) Other clonally related MAbs C989, C997, and C1035 from HV3-33/KV4-1 lineages that were found to beH3 specific with broad cross-reactivity (indicated by arrows).