FIG 2.

Effect of SFTSV infection on synchronized cells. (A) HepG2 cells were blocked at the G₀/G₁, S, or G₂/M phase by growth in serum-free culture, culturing with 0.85 mM thymidine (Thymi), or culturing with 50 ng/ml nocodazole (Noco), respectively, for 24 h. (B) Cell synchronization experimental workflow. (C, D, and E) HepG2 cells were synchronized at different phases, infected with SFTSV (10 MOI), and then cultured in fresh medium for another 48 h. The cell cycle distribution was analyzed by flow cytometry (*, P < 0.05; ***, P < 0.001).