FIG 4.

The SFTSV nonstructural protein (NSs) mediates cell cycle modulation. (A) HeLa cells were transfected with plasmids encoding VR1012, NSs-HA, NP-HA, Gc-HA, Gn-HA, or L-HA. The cells were collected at 48 h, and the cell cycle distribution was analyzed by flow cytometry. (B) Detection of protein expression by Western blotting. (C) HeLa cells were transfected with plasmids encoding VR1012, NSs-HA, or NP-HA for 48 h, after which the cells were collected, fixed, permeabilized, blocked, and incubated with anti-HA antibodies. Flow cytometry was used to detect cells expressing the exogenous proteins and analyze their cell cycle distribution (*, P < 0.05).