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. 2020 Feb 28;94(6):e01575-19. doi: 10.1128/JVI.01575-19

FIG 7.

FIG 7

The NSs-P66/69A-HA mutant does not induce cell cycle arrest. (A) HeLa cells were transfected with plasmids encoding VR1012, NSs-HA, or NSs-P66/69A-HA. After 48 h, the cells were fixed, permeabilized, blocked, and incubated with antibodies. Laser confocal microscopy was used to observe formation of IBs by wild-type or mutant NSs and colocalization of these proteins with CDK1. (B) 293T cells were transfected with plasmids encoding VR1012, NSs-HA, or NSs-P66/69A-HA. The cells were collected 48 h later, and the cell cycle distribution was analyzed by flow cytometry (n.s, P > 0.05; ***, P < 0.001). (C) 293T cells were transfected with plasmids encoding VR1012, NSs-HA, or NSs-P66/69A-HA. The cells were collected, and interactions between NSs and CDK1 were detected by immunoprecipitation.