Skip to main content
. 2020 Feb 28;94(6):e01575-19. doi: 10.1128/JVI.01575-19

FIG 8.

FIG 8

A nonfunctional CDK1 deletion mutant counteracts NSs-mediated inhibition of cell cycle progression. (A) 293T cells were transfected with plasmids encoding VR1012 or CDK1-Flag. The cells were collected 48 h later and analyzed by flow cytometry. (B) HeLa cells were transfected with plasmids encoding CDK1-Flag and NSs-HA, CDK1-T15A-Flag and NSs-HA, or CDK1-T161A-Flag and NSs-HA. After 48 h, the cells were fixed, permeabilized, blocked, and incubated with antibodies. Laser confocal microscopy was used to observe the colocalization of NSs with wild-type or mutant CDK1. (C) 293T cells were transfected with plasmids encoding VR1012 alone, VR1012 and NSs-HA, CDK1-Flag and NSs-HA, CDK1-T15A-Flag and NSs-HA, or CDK1-T161A-Flag and NSs-HA. After 48 h, the cells were collected and analyzed by flow cytometry. (D) 293T cells were transfected with empty plasmid or plasmids encoding CDK1-Flag, CDK1-T15A-Flag, or CDK1-T161A-Flag. After 24 h, SFTSV (0.1 MOI) was allowed to adsorb to the 293T cells, and the cells were incubated for another 48 h. The cells were then collected for qRT-PCR analysis (***, P < 0.001).