TABLE 1.
Crystallographic data collection and refinement statistics
| Parameter | Value(s) for Fab 3G12-RSV G157–197b |
|---|---|
| PDB accession no. | 6UVO |
| Data collection statisticsa | |
| Space group | P3121 |
| Cell dimensions | |
| a, b, c (Å) | 139.33, 139.33, 94.77 |
| α, β, γ (°) | 90, 90, 120 |
| Resolution (Å) | 74.53–2.90 (3.00–2.90) |
| Total no. of reflections | 93,208 (14,475) |
| No. of unique reflections | 23,682 (3,763) |
| Rmergec | 0.097 (0.641) |
| I/σ(I) | 9.4 (1.9) |
| Completeness (%) | 99.5 (99.5) |
| Redundancy | 3.9 (3.8) |
| CC1/2d | 0.993 (0.601) |
| Refinement statistics | |
| Resolution (Å) | 74.53–2.90 |
| No. of reflections | 23,665 |
| Rwork/Rfreee | 0.193/0.209 |
| No. of atoms | |
| Protein | 3,595 |
| Ligand/ion | 0 |
| Water | 0 |
| B-factors (Å2) | |
| Protein: bnmAb | 62 |
| Protein: RSV G | 76 |
| Ligand/ion | 0 |
| RMSD | |
| Bond lengths (Å) | 0.015 |
| Bond angles (°) | 2.067 |
| Ramachandran plot (%) | |
| Favored regions | 95.7 |
| Allowed regions | 4.3 |
| Outliers | 0 |
a
Data from one crystal were used.
b
Values in parentheses are for the highest-resolution shell.
c
Rmerge = Σ|(I − ⟨I⟩)|/Σ(I), where I is the observed intensity.
d
CC1/2 is the Pearson correlation coefficient between random half-data sets.
e
Rwork = ΣǁFo| − |Fcǁ/Σ|Fo| for all data except 5%, which were used for Rfree calculation.