FIG 6.

Effect of CD80 expression on T cell exhaustion in the TG of latently infected mice. TG from latently infected HSV-CD80 [LAT(−)] and McKrae [LAT(+)] mice were individually isolated on day 28 p.i., and qRT-PCR was performed using total RNA as described in Materials and Methods. CD4, CD8, PD-1, Tim-3, IL-2, IL-21, TNF-α, and IFN-γ expression in naive mice was used as a baseline to estimate the relative expression of each transcript in TG of latently infected mice. GAPDH expression was used to normalize the relative expression of each transcript. Each point represents the mean ± SEM from 10 TG.