Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Primers used in BAC recombination
| Primer namea | Sequence (5ʹ–3ʹ) |
|---|---|
| M49-FLAG-F | ACAACACCGGCGCTGCCGGTCCGATCAACACTATTATCACTGAACTAAACATTATCACTGAACTAAACGACTACAAAGACCATGAC |
| M49-FLAG-R | TTAGTGAGTTTTGTCAGTTCAGAATTCCTTGTCATCGTCAAAAGTAGAGAGAGCTAGCTAGCTCGCTAGTTAGTGAGTTTTGTCAGTTCA |
| M49-dd-F | GCAGGCGCCCGCGCTTCCTCATCCCCATCGGCAATCGGACCCTGCGCTACCAATCGGACCCTGCGCTACGAATTCACCCGCAGCGGCTAC |
| M49-dd-R | CTACCCGGGTCTGAGGTCCGTCACTCCTTCACGTACAGGCGCGGCCGAGTTCCCGGAACCGCCGCCACCGCTACCCGGGTCTGAGGTCCG |
F, forward; R, reverse.
