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DP decreases the expression of VEGF and HIF-1a in HCC cells. (A) Immunofluorescence assays of VEGF and HIF-1a proteins in HepG2 and Hepa1-6 cells treated or untreated with 200 mg/L DP for 48 h. (B, C) WB analyses of VEGF and HIF-1a expression in HepG2cells treated or untreated with IGF-1 and DP. (D, E) WB analyses of VEGF and HIF-1a expression in Hepa1-6 cells treated or untreated with IGF-1 and DP. No significance (ns), *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
