Figure 3.

VEEV Nanoplasmid Vaccination Does Not Increase T Cell Responses following IM Delivery

Groups of female BALB/c mice were vaccinated with 5 μg of pWRG/VEEV or the various VEEV Nanoplasmids on days 0 and 21 by IM injection and then euthanized on day 28 for isolation of splenocytes. Splenocytes were stimulated with pools of 15-mer, overlapping peptides spanning the VEEV E1 or E2 proteins, and VEEV E1- or E2-specific (A and B) IFN-γ⁺ T cells and (C and D) IL-2⁺ T cells were quantified via ELISPOT. Data represent the group mean averages ± SEM. ∗∗∗∗p < 0.0001. p values were determined by one-way ANOVA with Tukey’s post hoc test.