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. Author manuscript; available in PMC: 2020 Apr 15.
Published in final edited form as: Int J Basic Clin Immunol. 2019 Sep 12;2:1–12.

Figure 2. IL-15 overexpression in the esophagus promotes in vivo B cell induction, activation and IgE production.

Figure 2.

IgE and IL-4 levels in the blood of IL-15 overexpressed mice were measured by ELISA analysis (A, B). Analysis of B cells and activated B cells in the esophagus of IL-15 transgenic DOX and No-DOX exposed mice was performed by flow cytometry analyses using fluorochrome conjugated anti-CD45-APC-Cy7, anti-B220-PE-Cy7, anti-CD69-PE and respective matched isotype controls antibodies (C). The absolute and activated B cells from 3 independent experiments are shown (D, E). Further, we also examined whether IL-15-induced B cell activation is independent to IL-4; therefore, the spleenocytes of WT mice B cells were cultured in the presence of various concentrations of IL- 15 and IL-4. The activation of B cells was analyzed by flow cytometer (F). Data are expressed as mean ± SD, n = 6 mice/group. Data are expressed as mean ± SD, n = 10 mice/group, p<0.05, p<0.01, p<0.006.