Table 1.
Spike-Recovery Results from Single and Multiplex Immunoassays in Human Serum
| method | biomarker protein | spiked concentrations (pg/mL) | found concentrationsa (pg/mL) | (%) recovery (±SD) |
|---|---|---|---|---|
| cell-phone-assisted single tip assay | VEGF | 100 | 107 (±6) | 107 (±7) |
| 1000 | 950 (±84) | 95 (±9) | ||
| 4000 | 4133 (±454) | 103(±7) | ||
| colorimetric eight-chamber tip assay | PSA | 20 | 21 (±1.5) | 105 (±7) |
| 300 | 296 (±38) | 98 (±13) | ||
| 2000 | 2397 (±144) | 119 (±6) | ||
| VEGF | 125 | 122 (±15) | 98 (±12) | |
| 500 | 436 (±42) | 87 (±9) | ||
| 2000 | 2051 (±287) | 102 (±14) | ||
| IGF-1 | 125.0 | 121 (±16) | 100 (±13) | |
| 800 | 925 (±138) | 98.5 (±15) | ||
| 2000 | 2134 (±320) | 93 (±15) | ||
| CD-14 | 10 | 11 (±0.89) | 113 (±7) | |
| 500 | 450 (±43) | 90 (±9) | ||
| 5000 | 5402 (±486) | 108 (±9) |
a
After subtraction of the control human serum concentration.