Table 2.

Comparison between ELISA in a Tip vs. Microplate ELISA

comparison type	tip ELISA (colorimetry)	tip ELISA (chemiluminescence)	tip ELISA (cell phone)	traditional ELISA
assay time
sample incubation	30 min	30 min	30 min	150 min
detection antibody	20 min	20 min	20 min	60 min
streptavidin–HRP	15 min	15 min	15 min	45 min
development and quench	12 min	12 min	12 min	32 min
detection	2 min	2 min	3–5 s	2 min
approx. total wash time	6 min	6 min	6 min	9 min
total assay time	85 min	85 min	83 min	298 min
reagent/sample volume	50 μL for sample and wash	50 μL for sample and wash	50 μL for sample and wash	150 μL for sample and 300 μL for wash
LOD (pg/mL) and dynamic range (pg/mL)	PSA - 5 and 5 – 10 000 VEGF - 25 and 50 – 10 000	PSA 1 and 1 – 100 000	IGF-1 20 and 20 – 5500	PSA - 900 and 900 – 60 000 VEGF - 25 and 35 – 2000
	IGF-1 4 and 4 – 8000 CD-14 - 0.5 and 1 – 10 000		VEGF 50 and 50 – 10 000	IGF-1 - 100 and 100 – 8000 CD-14 - 62 and 62 – 4000
assay cost per microwell	$1.20/single tip	$1.20/single tip	$1.20/single tip	$4.14/microwell (Human PSA ELISA Kit – $398)
required instrument	plate reader	CCD camera	smart phone	no
operator	professional	simple training	simple training	professional
multiplexity	yes	yes	yes	no