Figure 3.
In vivo uptake of rhCTSD by CTSD-deficient mice. A Scheme of the intravenous injection (i.v.) of 25 mg/kg rhCTSD in the tail vein of P20 ctsd-/- mice and sacrifice for organ collection after different time points (2–48 h). B Immunoblot of CTSD plasma levels at different time points after rhCTSD i.v. injection. C Immunoblot of CTSD protein levels and D CTSD activity (relative to WT) in the liver of ctsd-/- mice at different time points after rhCTSD i.v. injection. E Immunoblot of CTSD protein levels and F CTSD activity (relative to WT) in the brain of ctsd-/- mice at different time points after rhCTSD i.v. injection.