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. 2019 Jul 16;16(5):811–825. doi: 10.1080/15548627.2019.1637200

Figure 5.

Figure 5.

ERT with rhCTSD in murine hippocampal brain slices and retinas of CTSD-deficient mice. A Immunoblot of day in vitro (DIV) 10 hippocampal organotypic slices of ctsd-/- mice and controls treated with rhCTSD for 5 d. B Quantification of the LC3-II protein levels relative to GAPDH in organotypic brain slices treated with rhCTSD. C Quantification of the ratio LC3-II:LC3-I proteins in organotypic brain slices treated with rhCTSD. Data (n = 2-4, mean ± S.E.M.) were analyzed using an unpaired t-test. * P < 0.05, ** P < 0.01. D Immunohistochemistry showing CTSD and CD68 in the retina of PBS- (LA) and rhCTSD- (RA) injected eyes of P23 CTSD-deficient mice. Scale bar: 50 µm. E Quantification of the number of CD68-positive cells in the retina of PBS- and rhCTSD-injected CTSD-deficient eyes. Data (n = 5, mean ± S.E.M.) were analyzed using an unpaired t-test. * P < 0.05. F DAPI staining of WT and ctsd-/- retinas at P23 (i,ii). Indicated are the different cellular retinal layers: outer nuclear layer (onl), inner nuclear layer (inl), inner plexiform layer (ipl) and ganglion cell layer (gcl). Immunostaining of SAPD (iii, iv), ATP5G1 (v,vi), LAMP1 (vii,viii), LAMP2 (ix,x) and SQSTM1 (xi,xii) in the retina of PBS- (LA) or rhCTSD- (RA) injected eyes of P23 CTSD-deficient mice. Scale bar: 50 µm.