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HEK293 cells were transfected with plasmids expressing Lac Z, human IL1R1 (hIL1R1), Flag-tagged LepRb or hIL1R1 and Flag-tagged LepRb together. The experiments were repeated for two times with similar outcome. Representative immunoblots depict the immunoblotting results of IL1R1 and Flag in the Flag immunoprecipitates (top). The expression levels of IL1R1 and HSP90 in the input total cell lysates are shown in the lower panels.
