Table 2.
Comparative performances of RT-PCR and Real-time RT-qPCR assays for detection of aerosol specimens
| Herd No. | Size | PI positivity | Sero-positivity | Samples background | Number of samples | RT-PCR | Real-time qPCR | ||
|---|---|---|---|---|---|---|---|---|---|
| P | N | P | N | ||||||
| A | 700 | 2.4% | 37% | Milking parlour | 7 | 1 | 6 | 3 | 4 |
| Cowshed | 7 | 1 | 6 | 2 | 5 | ||||
| Cattle playground | 8 | 0 | 8 | 0 | 8 | ||||
| B | 168 | 1.2% | 24% | Milking parlour | 12 | 1 | 11 | 1 | 11 |
| Cowshed | 6 | 1 | 5 | 1 | 5 | ||||
| Cattle playground | 9 | 0 | 9 | 0 | 9 | ||||
| C | 306 | 3.0% | 43% | Milking parlour | 12 | 1 | 11 | 3 | 9 |
| Cowshed | 10 | 1 | 9 | 3 | 7 | ||||
| Cattle playground | 12 | 0 | 12 | 0 | 12 | ||||
| D | 209 | 2.9% | 27% | Milking parlour | 9 | 0 | 9 | 1 | 8 |
| Cowshed | 13 | 2 | 11 | 5 | 8 | ||||
| Cattle playground | 10 | 0 | 10 | 0 | 10 | ||||
| E | 525 | 3.5% | 69% | Milking parlour | 10 | 3 | 7 | 5 | 5 |
| Cowshed | 11 | 2 | 9 | 2 | 9 | ||||
| Cattle playground | 9 | 0 | 9 | 0 | 9 | ||||
| F | 326 | 1.3% | 45% | Milking parlour | 8 | 1 | 7 | 2 | 6 |
| Cowshed | 8 | 1 | 7 | 1 | 7 | ||||
| Cattle playground | 8 | 0 | 8 | 0 | 8 | ||||
| Total | 169 | 15 | 154 | 29 | 140 | ||||
P Positive, N Negative