Figure 2.

Effect of exogenous CRH (A), CRHR1-specific agonist stressin 1 (C) and CRHR2-specific agonist UCN2 (E) on dynamic insulin secretion from isolated, perifused female mouse islets. Islets were exposed to physiological buffer containing 20 mmol/L glucose only or supplemented with agonists between 30 and 50 min. All CRHR agonists potentiated glucose-stimulated insulin secretion over that seen from control islets, as demonstrated by the rate of insulin secretion (A, C, E) and area under curve data (B, D, F). Data are presented as mean ± s.e.m., n = 3–4 per treatment group, AUC 20 mmol/l glucose + agonist, 30–50 min, *P < 0.05, **P < 0.01, ***P < 0.001; Students t-test control vs agonist treatment.