Figure 4.

Chromatin immunoprecipitation assay of the binding of GABPA to the DICER1 promoter. (A) Gel electrophoresis showing DNA bands from DICER1 promoter PCR product. Lane 1 shows band from 2% input DNA, lane 2 shows band from pulled down DNA using GABPA antibody and lane 3 shows PCR product from cross-linked chromatin DNA incubated with normal rabbit IgG. (B) Chromatogram from the GABPA antibody pulled down DNA and subsequent amplification and sequencing of the DICER1 promoter using the reverse primer as sequencing primer. The sequence was blasted with a 100% match in the DICER1 promoter. A full colour version of this figure is available at https://doi.org/10.1530/ERC-19-0446.