Fig. 3.

Synergistic effect of arachidonic acid (AA), eicosapentaenoic acid (E), lipoxin A4 (L) and BDNF (B) on chemical-induced cytotoxicity (AL/STZ/DB/BP) to RIN cells in vitro. a) Synergistic effect of AA and BDNF on AL/STZ/DB/BP-induced cytotoxicity to RIN5F cells in vitro., **P ≤ 0.001; ₰ P ≤ 0.01 § P < 0.05 vs untreated control; *P < 0.001 respective treated group vs toxin (T) (i.e., T vs T + AA10; T vs T + B50; T vs T + AA10 + B50); #P ≤ 0.01 vs individual and respective treated group (i.e., AA10 vs T + AA10; B50 vs T + B50; AA10 + B50 vs T + AA10 + B50) and ^aP ≤ 0.05 vs treated and synergistic combination group (i.e., T + AA10 vs T + AA10 + B50; T + B50 vs T + AA10 + B50). b) Synergistic effect of eicosapentaenoic acid (E) and BDNF on AL/STZ/DB/BP-induced cytotoxicity to RIN5F cells in vitro, **P ≤ 0.001; ₰P ≤ 0.01 § P ≤ 0.05 vs untreated control; *P < 0.001 vs respective toxin treated group; ^aP ≤ 0.01 vs respective individual treated group (i.e., E10 Vs T + E10; B50 Vs T + B50; E10 + B50 vs T + E10 + B50) and ^#P ≤ 0.05 vs treated and synergistic combination group (T + B50 Vs T + E10 + B50). c) Synergistic effect of lipoxinA4 (L) and BDNF (B) on AL/STZ/DB/BP-induced cytotoxicity to RIN5F cells in vitro., **P ≤ 0.001; ₰P ≤ 0.05;*P ≤ 0.01 vs untreated control; §P < 0.001 vs respective toxin treated group; ^#P ≤ 0.01 vs respective individual treated group (i.e. L50 vs T + L50; B50 vs T + B50; L50 + B50 vs T + L50 + B50) and ^aP ≤ 0.05 vs treated and synergistic combination group (T + L50 vs T + L50 + B50; T + B50 vs T + L50 + B50). All the above set of experiments were done in triplicate on three separate occasions (n = 9) and all values expressed as mean ± SEM