Fig. 5.

Measurement of LXA4 in the supernatants of RIN5F cells. a) Measurement of LipoxinA4 (ng/ml) in the supernatants of RIN5F cells treated with AL (6 mM)/ BP (1.5 mM)/STZ (20 mM)/DB (100 ng) ± AA (15 μg/ml). All values expressed as mean ± SEM. § P < 0.001 vs untreated control, and * P < 0.001 vs toxin-treated group. b) Measurement of LipoxinA4 (ng/ml) in the supernatants of RIN5F cells treated with AL (6 mM)/BP (1.5 mM)/STZ (20 mM)/DB (100 ng) ± BDNF (100 ng/ml). All values expressed as mean ± SEM. § P < 0.001 vs untreated control, and *P < 0.001 vs toxin-treated group. c) Measurement of LipoxinA4 (ng/ml) in the supernatants of RIN5F cells following synergistic treatment with sub-optimal dose of Arachidonic acid (AA-10 μg/ml) and BDNF (50 ng/ml) ± AL(6 mM)/BP(1.5 mM)/STZ(20 mM)/DB(100 ng/ml). All values expressed as mean ± SEM. * P < 0.01 vs untreated control; ₰P < 0.001; § P < 0.05 vs toxin group; ^aP ≤ 0.01 vs individual and respective treated groups (i.e., AA10 vs T + AA10; B50 vs T + B50; AA10 + B50 vs T + AA10 + B50) and ^#P ≤ 0.05 vs treated and synergistic combination group (i.e., T + AA10 vs T + AA10 + B50; T + B50 vs T + AA10 + B50). All the above set of experiments were done in triplicate (n = 3) and all values expressed as mean ± SEM. T = Toxin (AL/STZ/BP/DB)