Table 2.

Summary of analysis of various anti-oxidants in RIN5F treated with Alloxan(Pre-treatment)/STZ (Sim-treatment) and sub-optimal doses of AA (10 μg/ml) and BDNF (50 ng/ml)

SUMMARY OF THE ANALYSIS OF VARIOUS ANTI-OXIDANT ENZYMES IN RIN5F CELLS TREATED WITH AL/STZ
CHEMICAL	GROUP	SOD (Units/mg protein)	CAT (Mm H2O2/min/mg protein	GST (mM/min/gm protein)	GPX (mM/min/gm protein)
ALLOXAN	CONTROL	23.6 ± 1.7	466 ± 52.1	54.75 ± 1.4	739.8 ± 37.2
	AL(6 mM)	39.88 ± 3.5§	739.1 ± 45§	56.85 ± 3.2§	1649 ± 81.6§
	AA(10 μg/ml)	19.21 ± 0.9	407.7 ± 36.5	47.74 ± 1.8	534.79 ± 24.4
	BDNF(50 ng/ml)	19.12 ± 1.4	384.4 ± 30.9	41.44 ± 2.2	556 ± 18.7
	AA + BDNF	21.58 ± 2.6	326.7 ± 24.9	38.79 ± 2.1	560 ± 16.6
	AL + AA(10 μg/ml)	21.28 ± 2.8	356 ± 67	44 ± 0.86*	495.3 ± 7.4
	AL + BDNF	26 ± 1.43#	459 ± 76.2#	45.5 ± 0.8#	503.6 ± 3.5#
	AL + AA + BDNF	29.25 ± 0.7	432.3 ± 60	54.4 ± 2.23	768 ± 23.6
STREPTOZOTOCIN	CONTROL	24 ± 1.6	503.9 ± 45.2	54.5 ± 0.1	701 ± 19.4
	STZ(20 mM)	39.76 ± 1.4§	791 ± 86.1§	78.5 ± 0.76 §	1036 ± 38.3§
	AA(10 μg/ml)	21 ± 1.2	446 ± 49.3	51.9 ± 0.2	584 ± 23.1
	BDNF(50 ng/ml)	20.6 ± 1.3	420 ± 41.8	45.5 ± 0.42	581 ± 30.1
	AA + BDNF	24.1 ± 3.1	366 ± 32.2	43.7 ± 0.16	550 ± 38.6
	STZ + AA(10 μg/ml)	25 ± 7.8	528 ± 67	48.2 ± 0.96	505.3 ± 7.4
	STZ + BDNF	32 ± 1.7#	559 ± 86.2#	50.5 ± 0.2#	603.6 ± 3.5#
	STZ + AA + BDNF	34 ± 1.08	656 ± 57.1	54.5 ± 0.08	752.1 ± 21.4

Superoxide dismutase (SOD) is expressed as U SOD/mg of protein; Catalase (CAT) is expressed as μM of H₂O₂ consumed/minute/mg of protein; Glutathione-S-transferase (GST) is expressed as μM conjugate formed/ minute/gm of protein; Glutathione peroxidase (GPX) is expressed as μg of glutathione consumed/minute/gm of protein. ₰P < 0.001, § P ≤ 0.01 Vs Untreated control and * P < 0.01,# P ≤ 0.05, ** P < 0.001 Vs AL/STZ -treated group