Table 4.
Summary of analysis of various anti-oxidants in RIN5F treated with Benzopyrene(Pre-treatment)/DB(Sim-treatment) and sub-optimal doses of AA(10 μg/ml) + BDNF(50 ng/ml)
| SUMMARY OF THE ANALYSIS OF VARIOUS ANTI-OXIDANT ENZYMES IN RIN5F CELLS TREATED WITH DB/BP | |||||
|---|---|---|---|---|---|
| CHEMICAL | GROUP | SOD (Units/mg protein) | CAT (Mm H2O2/min/mg protein | GST (mM/min/gm protein) | GPX (mM/min/gm protein) |
| DOXORUBICIN | CONTROL | 34.6 ± 0.8 | 369 ± 58.6 | 44.75 ± 3.4 | 686.8 ± 27.2 |
| DB(100 ng/ml) | 29.2 ± 1.5§ | 689.1 ± 28§ | 51.65 ± 2.2§ | 1845 ± 69§ | |
| AA(10 μg/ml) | 31.2 ± 0.4 | 414.6 ± 26.5 | 37.74 ± 1.8 | 519 ± 14.8 | |
| BDNF(50 ng/ml) | 21 ± 0.9₰ | 404 ± 29.9 | 38.84 ± 2.9 | 601 ± 32.4 | |
| AA + BDNF | 29.5 ± 2.9 | 386 ± 32 | 32.49 ± 2.1 | 515 ± 21.4 | |
| DB + AA(10 μg/ml) | 30.6 ± 1.8 | 323 ± 41 | 39.3 ± 1.2 | 504 ± 5.4 | |
| DB + BDNF | 21 ± 2.8# | 409 ± 58.2 | 41.2 ± 0.8# | 698 ± 5.9# | |
| DB + AA + BDNF | 32.25 ± 0.3 | 414.3 ± 58 | 44.4 ± 2.28 | 727 ± 33 | |
| BENZOPYRENE | CONTROL | 44 ± 1.6 | 515.9 ± 49 | 44.5 ± 0.1 | 791 ± 29 |
| BP(1.5 mM) | 31.2 ± 1.8§ | 801 ± 61§ | 82.34 ± 1.2 § | 997 ± 41.3§ | |
| AA(10 μg/ml) | 19 ± 1.2 | 487 ± 57 | 48.9 ± 0.8 | 504 ± 33.3 | |
| BDNF(50 ng/ml) | 18.6 ± 1.7 | 402 ± 24.9 | 49 ± 0.62 | 506 ± 39.3 | |
| AA + BDNF | 22 ± 3.1 | 387 ± 38.2 | 41.7 ± 0.1 | 567 ± 39.1 | |
| BP + AA(10 μg/ml) | 24 ± 8.2 | 514 ± 39.8 | 51.2 ± 1.2 | 798.3 ± 2.6 | |
| BP + BDNF | 31 ± 2.1# | 498 ± 74.8# | 48.5 ± 0.2# | 799.6 ± 3.5# | |
| BP + AA + BDNF | 39 ± 0.67 | 508 ± 57.1 | 52.5 ± 0.08 | 698.1 ± 17.8 | |
Superoxide dismutase (SOD) is expressed as U SOD/mg of protein; Catalase (CAT) is expressed as μM of H2O2 consumed/minute/mg of protein; Glutathione-S-transferase (GST) is expressed as μM conjugate formed/ minute/gm of protein; Glutathione peroxidase (GPX) is expressed as μg of glutathione consumed/minute/gm of protein. ₰ P < 0.05, § P ≤ 0.01 Vs Untreated control and * P < 0.01, # P ≤ 0.05, ** P < 0.001 Vs DB/BP -treated group