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. 2020 Apr 15;15(4):e0220163. doi: 10.1371/journal.pone.0220163

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© 2020 Christensen et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 3 — MSCs differentiated using hPL-PIPC are shown in A, C, and E, while MSCs differentiated using hPL-PIPL are shown in B, D, and F. A and B show Alizarin Red S staining, used to demonstrate mineralization (black arrows) after 28 days of stimulation in osteogenic medium. C and D show Oil Red O staining, used to demonstrate accumulation of lipid droplets (black arrows) after 14 days of stimulation in adipogenic medium. E and F show Masson’s trichrome staining, used to demonstrate collagen fibers (black arrows) and lacunae formation (yellow arrows) after 35 days of chondrogenic stimulation.