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. 2020 Apr 3;14(4):e0008218. doi: 10.1371/journal.pntd.0008218

Fig 2. Relative expression of TGF-β/Smad signaling pathway key genes in goat PBMCs stimulated by the ESPs.

Fig 2

Cells were incubated with the serial concentration of ESP for 48 h and the mRNAs encoding TGF-β/Smad signaling pathway key genes a reverse-transcription-polymerase chain reaction quantified TGF-β1, TGF-βRI, TGF-βRII, Smad3, Smad4, Smad7, PU.1 and IRF-4. The significant level were set at *p < 0.05, **p < 0.01, ***p < 0.001 and ns non-significant compared with the untreated group (control). Data are representative of three independent experiments.