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. 2020 Apr 3;14(4):e0008218. doi: 10.1371/journal.pntd.0008218

Fig 5. Dot plot analysis (gated on CD2+CD4+ T cells) and proportion of Th9 cells during different stages of H. contortus by flow cytometry using representative intracellular cytokine antibodies (IL-9 and IL-10).

Fig 5

(a1): Dot plot analysis of trial 1; (a2): Th9 cells proportion of trial 1; (b1): Dot plot analysis of trial 2; (b2): Th9 cells proportion of trial 2. PBMCs derived-T helper-9 cells were collected from different groups of H. contortus infected dose rate. P-800: Goats (n = 10 each) infected with 800 H. contortus L3; P-2400: Goats infected with 2400 H. contortus L3; P-8000: Goats infected with 8000 H. contortus L3; 0–35: Days post-infection. The control group was kept untreated. Data are presented two experiment of the standard deviation (SD) and representative of triplicate experiments (*P < 0.05, **P < 0.01, ***P < 0.001).