Table 2.
Results of in-situ hybridisation, RT-PCR, and NASBA for H5 detection assay in selected brain regions of patient 2
|
ISH* |
RT-PCR |
NASBA-based H5 detection |
|||
|---|---|---|---|---|---|
| Sense | Antisense | Optical density (405 nm) | Result | ||
| Parietal lobe | + | + | + | 2·424 | + |
| Frontal lobe | − | − | + | 2·499 | + |
| Hippocampus | + | + | + | 2·521 | + |
| Occipital lobe | + | + | + | 2·312 | + |
| Internal capsule | + | + | + | 2·290 | + |
| Temporal lobe | + | + | + | 2·466 | + |
| Amygdala | + | + | + | 1·919 | + |
| Thalamus | + | + | + | 2·295 | + |
| Mesencephalon | − | − | − | 1·490 | + |
| Pons | + | + | + | 2·031 | + |
| Medulla oblongata | − | − | − | 2·469 | + |
ISH=in-situ hybridisation.
*
Signals obtained with sense and antisense probes were mainly located in cytoplasm of infected neurons.