Table 3.
Results of RT-PCR, strand-specific RT-PCR, real-time RT-PCR, and NASBA for H5 detection in specific tissue samples
|
RT-PCR and strand-specific RT-PCR |
Real-time RT-PCR |
NASBA-based H5 detection assay |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
|
Patient 1 |
Patient 2 |
Fetus |
Patient 1 |
Patient 2 |
|||||||
| +/− | − | + | +/− | − | + | Optical density (405 nm)* | Result | Optical density (405 nm)* | Result | ||
| Lungs | Y | Y | N | Y | N | Y | Y | 2·579 | Y | 0·961 | Y |
| Trachea | .. | .. | .. | Y | Y | Y | .. | .. | n/a | 1·236 | Y |
| Intestines | Y | Y | N | Y | Y | Y | N | 2·277 | Y | 2·112 | Y |
| Brain | .. | .. | .. | Y | Y | Y | .. | .. | n/a | 2·424 | Y |
| Heart | Y | N | Y | Y | N | N | N | 2·415 | Y | 2·555 | Y |
| Spleen | Y | Y | N | Y | N | N | .. | 1·326 | Y | 1·442 | Y |
| Liver | Y | N | N | Y | N | N | Y | 2·176 | Y | 2·225 | Y |
| Kidneys | Y | Y | N | Y | N | N | N | 2·371 | Y | 1·587 | Y |
| Lymph node | .. | .. | .. | N | Y | N | .. | .. | n/a | 1·107 | Y |
| Placenta | Y | Y | Y | n/a | n/a | n/a | n/a | 1·653 | Y | n/a | n/a |
+/−, −, and + represent total, negative-stranded, and positive-stranded RNA, respectively. Y=positive result. N=negative result. n/a=not applicable.
*
Samples regarded as H5-positive if absorbance higher than 0·45.