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. 2016 Mar 22;106(2):144–159. doi: 10.1002/bip.22808

Figure 3.

Figure 3

Flowchart for the generation of the two‐stranded α‐helical coiled‐coil template and conjugation of the template to the protein carrier (see Methods). nL denotes norleucine; DTDP, 2,2'‐dithiodipyridine; KLH, keyhole limpet hemocyanin; Traut's reagent, 2‐iminothiolane hydrochloride.