Figure 4.

ETX001 enhances fluid secretion in cystic fibrosis–human bronchial epithelial (HBE) cells under static and shear stress–stimulated conditions. (A) A cartoon illustrating the physiological regulation of anion secretion in the human airway epithelium. (B) Sample XZ confocal images of airway surface liquid (ASL) visualized using Texas red staining in the absence and presence of ETX001 (1 μM). (C) The change in ASL height from baseline was measured following a 1-hour treatment with ETX001 or vehicle under static conditions (i.e., no shear stress) and after 3 hours of continual shear stress. Data are expressed as the mean change in ASL height (±SEM) for seven to eight inserts from a F508del/F508del HBE cells donor and is representative of three independent experiments. Cystic fibrosis–HBE cells had not been pretreated with IL-13. *P < 0.001 and **P < 0.00001 for ETX001-treated versus time-matched vehicle control using two-tailed Student’s t test. TMEM16A = anoctamin-1.