Table 2.
Monoclonal antibodies used for immunohistochemical staining
| Antibody | Specificity | Cell type/chemokine | Titer | Source |
|---|---|---|---|---|
| OKT6 | CD1a | Langerhans cells | 1:25 | Dept. of Immunology, EMCR, Netherlands |
| T3‐4B5 | CD3 | Total T‐cell pool | 1:100 | DAKO, Denmark |
| Leu2a | CD8 | Cytotoxic T cells | 1:100 | B & D, Dorset, UK |
| Mon/1 | CD14 | Monocytes | 1:600 | CLB, Amsterdam, Netherlands |
| 80H5 | CD15 | Neutrophils | 1:25 | Immunotech, Marseille, France |
| IOB4a | CD19 | B cells | 1:200 | Immunotech, Marseille, France |
| EBM11 | CD68 | Macrophages | 1:300 | DAKO, Denmark |
| HP‐3B1 | CD94 | Natural killer (NK) cells | 1:50 | Coulter, Netherlands |
| G3 | tryptase | Mast cells | 1:100 | Chemicon, UK |
| BMK‐13 | MBP | Eosinophils | 1:100 | Sanbio, Uden, Netherlands |
| αEotaxin | Eotaxin | Chemokine | 1:15 | R & D systems, UK |
| αRANTES | RANTES | Chemokine | 1:50 | Chemicon, UK |
MBP: major basic protein.