Figure 2.

Breast regression protein‐39 (BRP‐39) expression in respiratory syncytial virus (RSV)‐infected murine model. C57BL/6 wild‐type (WT) mice were infected with RSV (4 × 10⁷ plaque‐forming units/mouse) via the intratracheal route. A,B, BRP‐39 mRNA level was increased in RSV‐infected mice at 7 dpi. C,D, BRP‐39 expression in bronchoalveolar lavage fluid and lung lysates was increased by RSV infection, as determined by ELISA. E, Immunohistochemical (IHC) detection of BRP‐39 in lung tissue sections (red arrows, airway epithelial cells; blue arrows, alveolar macrophages). F, Double‐labeled IHC was performed to identify alveolar macrophages producing BRP‐39 (orange box). G, Enlarged image of the orange box shown in Figure 2F (white arrows, BRP‐39 producing alveolar macrophages). Values in B, C, and D represent mean ± SD of at least three independent experiments. n.d., not detected. **P < 0.01, ***P < 0.001 [Colour figure can be viewed at http://wileyonlinelibrary.com]