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. 2009 Jan 19;48(8):1378–1398. doi: 10.1002/anie.200802092

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Copyright © 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim

This article is being made freely available through PubMed Central as part of the COVID-19 public health emergency response. It can be used for unrestricted research re-use and analysis in any form or by any means with acknowledgement of the original source, for the duration of the public health emergency.

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Creation of replication‐deficient viral vectors. For gene transfer, the central protein‐coding genes of the viral genome are removed and replaced with the transgene or an shRNA expression cassette. The vector genome is packaged in a packaging cell line expressing the viral genes, which in most cases are spread over several plasmids. The resulting virus vector only includes the expression cassette for the transgene, while the essential virus genes are missing, so that further replication is impossible.