Table 2.
Primers and probes
| Primers and probes | Oligonucleotide sequence (5′‐3′) | Target gene |
|---|---|---|
| Inf A F | GAC CRA TCC TGT CAC CTC TGA C | M |
| Inf A R | AGG GCA TTY TGG ACA AAK CGT CTA | |
| Inf A P | FAM‐TGC AGT CCT CGC TCA CTG GGC ACG‐BHQ1 | |
| Inf B F | TCC TCA AYT CAC TCT TCG AGC G | NC |
| Inf B R | CGG TGC TCT TGA CCA AAT TGG | |
| Inf B P | 6FAM‐CCA ATT CGA GCA GCT GAA ACT GCG GTG‐BHQ1 | |
| PIV1 F | GTT GTC AAT GTC TTA ATT CGT ATC AAT AAT T | HN |
| PIV1 R | GTA GCC TMC CTT CGG CAC CTA A | |
| PIV1 P | FAM‐TAG GCC AAA GAT TGT TGT CGA GAC TAT TCC AA‐BHQ1 | |
| PIV2 F | GCA TTT CCA ATC TTC AGG ACT ATG A | HN |
| PIV2 R | ACC TCC TGG TAT AGC AGT GAC TGA AC | |
| PIV 2 P | CAL FO560‐CCA TTT ACC TAA GTG ATG GAA TCA ATC GCA AA‐BH | |
| PIV 3 F | CCA GGG ATA TAY TAY AAA GGC AAA A | HN |
| PIV 3 R | CCG GGR CAC CCA GTT GTG | |
| PIV 3 P | FAM‐TGG RTG TTC AAG ACC TCC ATA YCC GAG AAA‐BHQ1 | |
| ADV F | GCC CCA GTG GTC TTA CAT GCA CAT C | Hexon |
| ADV R | GCC ACG GTG GGG TTT CTA AAC TT | |
| ADV P | FAM‐TGC ACC AGA CCC GGG CTC AGG TAC TCC GA‐BHQ1 | |
| RSV F | AAT ACA GCM AAA TCT AAC CAA CTT TAC A | L |
| RSV R | GCC AAG GAA GCA TGC AAT AAA | |
| RSV P1 | FAM‐TGC TAT TGT GCA CTA AAG‐BHQ1 | |
| RSV P2 | CAL FO560‐CAC TAT TCC TTA CTA AAG ATG TC‐BHQ1 | |
| hMPV F | CATATAAGCATGCTATATTAAAAGAGTCTC | NS |
| hMPV R | CCTATTTCTGCAGCATATTTGTAATCAG | |
| hMPV P | FAM‐TGY AAT GAT GAG GGT GTC ACT GCG TGG G‐BHQ1 | |
| CMV F | CCG GCA AGC TCT TTA TGC A | Phosphoprotein 65 |
| CMV R | TGG GAC ACA ACA CCG TAA AGC | |
| CMV P | FAM‐CCG CAA CCC TTC AT‐BHQ1 | |
| RV F | GCA CTT CTG TTT CCC C | 5′ noncoding region |
| RV R | GGC AGC CAC GCA GGC T | |
| RV P1 | FAM‐AGC CTC ATC TGC CAG GTC TA‐BHQ1 | |
| RV P2 | CAL FO560‐AGC CTC ATC CAC CAA ACT A‐BHQ1 | |
| hBoV F | TGC AGA CAA CGC YTA GT TGT TT | NS1 |
| hBoV R | CTG TCC CGC CCA AGA TAC A | |
| hBoV P | 6FAM‐CCA GGA TTG GGT GGA ACC TGC AAA‐BHQ1 | |
| OC43 + HKU1 | Polymerase 1b | |
| CoV F1 | TGG TGG CTG GGA CGA TAT GT | |
| CoV R1 | GGC ATA GCA CGA TCA CAC TTA GG | |
| CoV P1 | 6‐FAM‐ATA ATC CCA ACC CAT RAG‐BHQ1 | |
| NL63 | Polymerase 1b | |
| CoV F2 | TTT ATG GTG CTT GGA ATA ATA TGT TG | |
| CoV R2 | GGC AAA GCT CTA TCA CAT TTG G | |
| CoV P1 | FAM‐ATA ATC CCA ACC CAT RAG‐BHQ1 | |
| 229E | Polymerase 1b | |
| CoV F3 | TGG CGG GTG GGA TAA TAT GT | |
| CoV R3 | GAG GGC ATA GCT CTA TCA CAC TTA GG | |
| CoV P2 | CAL FO560‐ATA GTC CCA TCC CAT CAA‐BHQ1 | |
| RnaseP F | AGA TTT GGA CCT GCG AGC G | Human ribonuclease P |
| RnaseP R | GAG CGG CTG TCT CCA CAA GT | |
| RnaseP P | FAM‐GAG CGG CTG TCT CCA CAA GT‐BHQ1 |
F = forward; HN = hemagglutinin‐neuramidase; L = RNA polymerase large subunit; M = matrix; NC = nucleocapsid; NS = nonstructural gene; P = probe; R = reverse.