Table 3.
Classification of field submissions into subgroups of infectious bronchitis virus
| ID | HRM classification (subgroup) | GCP with subgroup reference | S1 gene length used to generate identities e | Nucleotide sequence identity with subgroup reference (%) | |
|---|---|---|---|---|---|
| S1 gene | 3′UTR | ||||
| 013 | 1 a | 95.66 | 1717 f | 99 | 100 |
| 015 | Unclassified b | NA d | 1723 f | Subgroup 2 h (93) | Subgroup 3 (98) |
| 018 | 3 c | 84.95 | 1705 f | 99 | 99 |
| 020 | 1 | 97.41 | − g | − | 99 |
| 025 | 1 | 93.32 | 1138 | 99 | 99 |
| 026 | 1 | 94.14 | 1717 f | 99 | 99 |
| 027 | 1 | 92.43 | 1684 | 99 | 99 |
| 028 | 1 | 87.94 | 1717 f | 99 | 99 |
| 031 | 1 | 95.86 | 1425 | 100 | 99 |
| 032 | 1 | 89.99 | 1666 | 99 | 99 |
| 038a | 1 | 86.19 | 1294 | 99 | 99 |
| 038b | Unclassified | NA | 1225 | Subgroup 1 (99) | Subgroup 1 (99) |
| 040 | 3 | 94.30 | 925 | 99 | 99 |
| 041 | 1 | 82.80 | − | − | 99 |
| 043 | Unclassified | NA | 800 | Subgroup 3 (99) | Subgroup 3 (99) |
| 044 | Unclassified | NA | 1717 f | Subgroup 1 (99) | Subgroup 1 (99) |
| 050 | Unclassified | NA | − | − | Subgroup 3 (99) |
a
Vaccine I used as the genotype/reference.
b
b HRM profile did not match any of the class reference strains.
c
c Reference strain N1/03 used as the genotype/reference.
d
d Highest GCP below cut‐off.
e
Maximum length of sequence obtained from S1 gene sequencing that was used to generate the nucleotide sequence identities (full amplicon approximately 1720 bp).
f
f Complete S1 gene sequence obtained.
g
g Amplification/sequencing not successful.
h
h Reference strain N1/88 used as the genotype/reference.
bp, base pairs; GCP, genotype confidence percentage; HRM, high‐resolution melt; NA, not available; UTR, untranslated region.