Figure 2. VE-cadherin dynamics is driven by ARP2/3 complex controlled JAIL formation.

Time-lapse recording of sub confluent HUVECs that co-express both VE-cadherin-mCherry and EGFP-p20, a component of the ARP2/3 complex. VE-cadherin remodeling is initiated by ARP2/3-controlled and actin-driven formation of JAIL (dotted line), which then overlap adjacent membranes allowing trans-dimer engagement of free floating VE-cadherin in the membrane (VE-cadherin plaques), followed by JAIL retraction that subsequently clusters and incorporates VE-cadherin into the junctions (reproduced from our previous work⁴¹ (Abu Taha A et al. Mol Biol Cell 2014, 25: 245-256) with permission).