Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Lrrk2) | Lrrk2; LRRK2 | NA | MGI:1913975 | |
| Genetic reagent (Mycobacterium tuberculosis) | Mtb; Erdman | Watson et al., 2015; Watson et al., 2012 | ||
| Genetic reagent (Mycobacterium leprae) | Mlep | National Hansen's Disease Program | ||
| Genetic reagent (Mus musculus) | Lrrk2 KO; C57BL/6-Lrrk2tm1.1Mjff/J | Jackson Labs | 16121 Lrrk2 KO | |
| Genetic reagent (Mus musculus) | Ifnar KO; B6(Cg)-Ifnar1tm1.2Ees/J | Jackson Labs | 28288 Ifnar1 KO | |
| Genetic reagent (Mus musculus) | Tfam HET | obtained from A. P. West TAMHSC | Tfam HET | doi: 10.1016/j.ajpath.2011.10.003 |
| Genetic reagent (Mus musculus) | Cgas KO; B6(C)-Cgastm1d (EUCOMM)Hmgu/J | obtained from A. P. West TAMHSC | cGAS KO | |
| Cell line (Mus musculus) | RAW 264.7 Lrrk2 Parental; WT | ATCC | ATCC SC-6003 | |
| Cell line (Mus musculus) | RAW 264.7 Lrrk2 KO | ATCC | ATCC SC-6004 | |
| Cell line (Mus musculus) | RAW 264.7 | ATCC | ATCC TIB-71 | Cell line maintained in the Watson lab |
| Cell line (Homo sapiens) | U937 | ATCC | ATCC CRL-1593.2 | Cell line maintained in the Watson lab |
| Antibody | anti-pSTAT1 Rabbit monoclonal | Cell Signaling | (Tyr701) (58D6) #9167 | (1:1000 WB) |
| Antibody | anti-STAT1 Rabbit monoclonal | Cell Signaling | (D4Y6Z) #14995 | (1:1000 WB) |
| Antibody | anti-pIRF3 Rabbit monoclonal | Cell Signaling | (Ser396) (4D4G) #4947 | (1:1000 WB) |
| Antibody | anti-IRF3 Rabbit monoclonal | Cell Signaling | (D83B9) #4302 | (1:1000 WB) |
| Antibody | anti-Beta tubulin Rabbit polyclonal | Abcam | ab15568 | (1:5000 WB) |
| Antibody | anti-pDRP1 Rabbit polyclonal | Cell Signaling | (Ser616) #3455 | (1:75 FC), (1:1000 WB) |
| Antibody | anti-DRP1 Rabbit monoclonal | Abcam | ab184247 | (1:1000 WB) (1:200 IF) |
| Antibody | anti-IFNB Rabbit polyclonal | PBL Assay Science | 32400–1 | (1:250 neutralizing) |
| Antibody | anti-PFAS Rabbit polyclonal |
Bethyl Laboratories | A304-219A | (1:200 IF) |
| Antibody | Anti-TOM20 Mouse monoclonal | Millipore, via A.P. West lab TAMHSC | MABT166 | (1:200 IF) |
| Antibody | anti-TFAM Rabbit polyclonal | Millipore, via A.P. West lab TAMHSC | ABE483 | (1:1000 WB) |
| Antibody | anti-VDAC Rabbit polyclonal | Protein Tech, via A.P. West lab TAMHSC | 55259–1-AP | (1:1000 WB) |
| Antibody | Goat anti Rabbit IgG | Licor | IRDye 800CW | (1:10000 WB) |
| Antibody | Goat anti Rabbit IgG | Licor | IRDye 680CW | (1:10000 WB) |
| Antibody | Goat anti Rabbit IgG AF 488 | Invitrogen | A32731 | (1:500 FC) |
| Sequence-based reagent | interferon stimulatory DNA; ISD | IDT | annealed in house | (1 ug/ml) |
| Sequence-based reagent | CpG 2395 | Invivogen | tlrl-2395 | (1 uM) |
| Peptide, recombinant protein | recombinant IFNB | PBL Assay Science | 12405–1 | (200 IU/mL) |
| Commercial assay or kit | Seahorse XF mito stress kit | Agilent | 103708–100 | |
| Commercial assay or kit | Direct-zol RNA mini prep | Zymo research | R2052 | |
| Commercial assay or kit | Mouse Cytokine Array | Eve Technologies | MD13 panel | |
| Chemical compound, drug | thioglycollate | Fisher Scientific | BD 211716 | |
| Chemical compound, drug | DMXAA | Invivogen | tlrl-dmx | (5 ug/ml) |
| Chemical compound, drug | urate | Sigma Aldrich | U2625 | |
| Chemical compound, drug | IMP | Sigma Aldrich | 57510 | |
| Chemical compound, drug | hypoxanthine | Sigma Aldrich | H9377 | |
| Chemical compound, drug | FCCP | Sigma Aldrich | C2920 | (50 uM) |
| Chemical compound, drug | CLO97 | Invivogen | tlrl-c97 | (100 ng/ml) |
| Chemical compound, drug | JC1 dye | Thermofisher | T3168 | (1 uM) |
| Chemical compound, drug | TMRE | Thermofisher | T669 | (25 ng/ml) |
| Chemical compound, drug | ATP | Invivogen | tlrl-atpl | (5 uM) |
| Chemical compound, drug | rotenone | Sigma Aldrich | R8875 | (2.5 uM) |
| Chemical compound, drug | mitoTEMPO | Santa Cruz Biotechnology | (CAS 1569257-94-8) | (200 uM) |