Table 1. List of reagents and resources.
| Reagents | Source | Identifier |
| Antibodies | ||
| AOX antiserum (custom raised in rabbit) | 21st Century Biochemicals, Marlborough, MA, USA | (42) |
| HIF-1α (C-Term) polyclonal antibody | Cayman Chemical | 10006421 |
| Anti-beta actin antibody [mAbcam 8226]—loading control (HRP) |
Abcam | ab20272 |
| Anti-rabbit IgG (H + L), HRP conjugate | Promega | W4011 |
| Anti-mouse IgG (H + L), HRP conjugate | Promega | W4021 |
| Lentiviral particles | ||
| Lentiviral Ciona intestinalis AOX | This paper | N/A |
| Lentiviral catalytic inactive (mutant) AOX | This paper | (20) |
| Chemicals | ||
| n-Propyl gallate (nPG, AOX inhibitor) | Sigma-Aldrich | P3130 |
| U46619 (prostaglandin H2/thromboxane A2 receptor agonist) |
Enzo Life Sciences, Exeter, UK | PG-023 |
| Sodium chloride (NaCl) | Sigma-Aldrich | 31434 |
| Potassium chloride (KCl) | Carl Roth, Karlsruhe, Germany | 5346.1 |
| Calcium chloride (CaCl2) | Carl Roth | CN93.1 |
| Magnesium chloride (MgCl2) | Carl Roth | KK36.1 |
| Sodium dihydrogen phosphate dihydrate (NaH2PO4·2H2O) |
Merck Millipore, Darmstadt, Germany | 1063421000 |
| Sodium hydrogen carbonate (NaHCO3) | Carl Roth | 8551 |
| d(+)-Glucose | Carl Roth | X997.1 |
| l-Aspartic acid potassium salt (l-aspartate) | Sigma-Aldrich | A6558 |
| Adenosine 5′-triphosphate magnesium salt, ATP | Sigma-Aldrich | A9187 |
| Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′- tetraacetic acid (EGTA) |
Sigma-Aldrich | 03777 |
| Hepes (PUFFERAN®) | Carl Roth | 9105.3 |
| Potassium hydroxide (KOH) | Carl Roth | 6751 |
| 1-Hydroxy-3-methoxycarbonyl −2,2,5,5-tetramethylpyrrolidine (CMH spin probe) |
Noxygen, Elzach, Germany | NOX-02.2 |
| Superoxide dismutase–polyethylene glycol (pSOD, PEG-SOD) |
Sigma-Aldrich | S9549 |
| 5,5′,6,6’-Tetrachloro-1,1′,3,3′-tetraethyl- imidacarbocyanine iodide (JC-1) |
ThermoFisher Scientific | T3168 |
| Penicillin-streptomycin | Gibco, ThermoFisher Scientific | 1507–063 |
| Normocin—antimicrobial reagent | InvivoGen, San Diego, USA | ant-nr-1 |
| Assays | ||
| ZytoChem Plus (HRP) Polymer Bulk Kit | Zytomed Systems GmbH, Berlin, Germany | POLHRP-100 |
| VECTOR NovaRED Peroxidase (HRP) Substrate Kit | Vector Laboratories, Burlingame, USA | SK-4800 |
| Enhanced chemiluminescence ECL Prime Western Blotting System |
GE Healthcare | RPN2232 |
| Cell Proliferation ELISA, BrdU (colorimetric) assay | Sigma-Aldrich | 11647229001 |
| Primary cells | ||
| Mouse PASMC from precapillary pulmonary arterial vessels |
(30) | N/A |
| Rat PASMC from precapillary pulmonary arterial vessels |
(45) | N/A |
| Rodent models | ||
| Mouse: Rosa26-Aox (aka AoxRosa26) | (10) | N/A |
| Mouse: C57BL/6J | Charles River, Sulzfeld, Germany | Strain Code 632 |
| Rat: Sprague-Dawley (Crl:SD) | Charles River | Strain Code 400 |
| Plasmid and vectors | ||
| pWPXL (constitutive lentiviral plasmid) | Addgene, Boston, MA, USA | Plasmid #12257 |
| pMD2.G (envelope vector) | Addgene | Plasmid #12259 |
| psPAX2 (packing vector) | Addgene | Plasmid #12260 |
| Software and algorithms | ||
| Patchmaster software | HEKA, Lambrecht, Germany | v2x90 |
| IGOR Pro software | Wavemetrics, Lake Oswego, OR, USA | version 6.37 |
| DatLab software | Oroboros Instruments, Innsbruck, Austria | DatLab 5 |
| Prism software | GraphPad Software; San Diego, CA, USA | version 8.2.0 |
| Other | ||
| Multi Wire Myograph System | Danish Myo Technology A/S, Aarhus, Denmark | 620 M |
| N2-balanced gas (21% O2, 5% CO2) | Praxair, Danbury, CT, USA | 592091 (custom made) |
| N2-balanced gas (1% O2, 5% CO2) | Praxair | 650329 (custom made) |
| Optical Oxygen Meter (FireStingO2) | Pyro Science, Aachen, Germany | FSO2–2 |
| Culture dishes | Greiner Bio-One, Frickenhausen, Germany | 627860 |
| Multi-line in-line solution heater | Warner Instruments, Hamden, CT, USA | SHM-8 |
| Borosilicate glass capillary tubes | Sutter Instrument, Novato, CA, USA | BF150–86-10HP |
| DMZ universal electrode puller | Zeitz, Martinsried, Germany | N/A |
| EPC 10 USB patch clamp amplifier | HEKA | 895000 |
| EMXmicro ESR spectrometer | Bruker Biospin GmbH, Rheinstetten, Germany | N/A |
| OROBOROS-2k oxygraph | Oroboros Instruments | N/A |
| Media 199—Mammalian Cell Culture | Gibco, ThermoFisher Scientific | 31150–022 |
| Vevo2100 high-resolution imaging system equipped with a 40-MHz transducer |
VisualSonics, Toronto, Canada | N/A |
| Raman spectrometer Shamrock 303i | Andor, Technology, Belfast, UK | SR-303i-A |
| Laser | Altechna, Vilnius, Lithuania | DPSS 532 |
| Raman filter cube with dichroic mirror | Semrock, Rochester, NY, USA | zt532/640rpc |
| 532-nm EdgeBasic best-value long-pass edge filter | Semrock | BLP01-532R-25 |
| ×40 magnification objective | Olympus, Tokyo, Japan | LUCPLFLN |