Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Mus musculus) |
Eomes | Eomes→ Tbr2 | ||
| Antibody | anti-Tbr2 (Rat monoclonal) |
Thermo Fisher Scientific | Cat# 14-4875-82, RRID:AB_11042577 | IF (1:250) |
| Antibody | anti-Tbr2 (mouse monoclonal) |
Thermo Fisher Scientific | Cat# 14-4877-80, RRID:AB_2572881 | IF (1:500) |
| Antibody | anti-Prox1 (rabbit polyclonal) |
EMD Millipore | Cat# ABN278, RRID:AB_2811075 | IF (1:500) |
| Antibody | anti-Sox9 (rabbit ployclonal) |
EMD Millipore | Cat# AB5535 | IF (1:1000) |
| Antibody | anti-PCNA (mouse monoclonal) |
EMD Millipore | Cat# MAB4076, RRID:AB_95029) | IF (1:500) |
| Antibody | anti-Hes1 (rabbit polyclonal) |
Gift from Dr. Nadean Brown (Nelson et al., 2013) | IF (1:1000) | |
| Antibody | anti-Pax6 (rabbit ployclonal) |
Biolgend (Covance) |
Cat# 901301 | IF (1:1000) |
| Antibody | anti-Pax6 (mouse monoclonal) |
DSHB | supernatent | IF (1:50) |
| Antibody | anti-NeuroD1 (goat polyclonal) |
Santa Cruz | Cat# (N-19): sc-1084 | IF (1:400) |
| Antibody | ranti-GFAP (rabbit polyclonal) |
Dako | Cat# GA524, RRID:AB_2811722 | IF (1:1000) |
| Antibody | anti-Nestin (mouse monoclonal) |
DHSB | RC2 | IF (1:50) |
| Antibody | anti-Histone H3 (phospho S28) (rat monoclonal) |
Abcam | Cat# ab10543 | IF (1:500) |
| Antibody | anti-Reelin (mouse monoclonal) |
EMD Millipore | Cat# MAB5364 | IF (1:1000) |
| Antibody | anti-GFP (chicken polyclonal) |
Abcam | Cat# ab13970, RRID:AB_300798 | IF (1:500) |
| Antibody | anti-Dll1 (sheep polyclonal) |
R and D | Cat# AF5026 | IF (1:100) |
| Antibody | anti-Dll3 (rabbit polyclonal) |
Santa Cruz | Cat# sc-67269 | IF (1:100) |
| Antibody | Donkey anti-rabbit ALEXA 488/568/647 | Thermo Fisher Scientific | Cat# R37118, A10042 , A-31573 |
IF (1:500) |
| Antibody | Donkey anti-rat ALEXA 488/594 |
Thermo Fisher Scientific | Cat# A-21208, A-21209 | IF (1:500) |
| Antibody | Donkey ant-mouse ALEXA 488/568/647 | Thermo Fisher Scientific | Cat# R37115, A10037, A-31571 | IF (1:500) |
| Antibody | Donkey anti-chicken ALEXA 488 |
Jackson | Cat# 703-545-155 | IF (1:500) |
| Antibody | Donkey anti-sheep ALEXA 568 |
Thermo Fisher Scientific | Cat# A-21099 | IF (1:500) |
| Chemical compound, drug | DAPI 4',6-diamidino-2- phenylindole | Invitrogen | Cat# D1306 | IF (1:10,000) |
| Other | Fluoromout-G | Southern Biotech | Cat# 0100–01 | |
| Chemical compound, drug | tamoxifen (TM) | Sigma | T5648 | |
| Other | NeuroBasal media | Invitrogen | Cat# 21103049 | |
| Other | N2 supplement | Invitrogen | Cat# 17502048 | |
| Other | B27 supplement | Invitrogen | Cat# 17504044 | |
| Other | Pen/Strep | Invitrogen | Cat# 15140122 | |
| Other | FBS | Invitrogen | Cat# 16000044 | |
| Other | HBSS | Invitrogen | Cat # 14025076 | |
| Genetic reagent Mus musculus |
EomesGFP | (Arnold et al., 2009) | Tbr2GFP KI | Knockin Tbr2GFP reporter |
| Genetic reagent Mus musculus |
Tg(Eomes-EGFP)DQ10Gsat | (Kwon and Hadjantonakis, 2007) | Tbr2GFP TG | BAC transgenic Tbr2GFP reporter |
| Genetic reagent Mus musculus |
Eomesflox/flox | (Intlekofer et al., 2008) | Tbr2 cKO | Cross to Cre lines for inducible and/or conditional knockout |
| Genetic reagent Mus musculus |
EomesCreERT2 | (Pimeisl et al., 2013) | Inducible CreT2 for tracking IP lineage | |
| Genetic reagent Mus musculus |
Gt(ROSA)26Ai14 | (Madisen et al., 2012) | Ai14 | lox-stop-lox tdTomato reporter |
| Genetic reagent Mus musculus |
Nes-GFP | (Mignone et al., 2004) | NesGFP | Transgenic NesGFP reporter |
| Genetic reagent Mus musculus |
Nes-Cre | (Tronche et al., 1999) | Transgenic NesCre driver | |
| Genetic reagent Mus musculus |
Nes-CreERT2 | (Imayoshi et al., 2006) | Transgenic NesCre driver | |
| Genetic reagent Mus musculus |
Hes5-GFP | (Basak and Taylor, 2007) | Hes5GFP | Transgenic Hes5GFP reporter |
| Other | FIJI | NIH | ||
| Other | Photoshop, Illustrator |
Adobe | ||
| Other | Imaris 7.4 | Bitplane | ||
| Other | Filmora9 | Wondershare | ||
| Other | Allen Developing Mouse Brain Atlas (2008) | http://developingmouse.brain-map.org/ |