Table 1.
Comparison of the Process of Enzymatic Digestion and Mechanical Digestion
| Enzymatic Digestion | Mechanical Digestion | |
|---|---|---|
| Isolation methods | ||
| Addition of the digestive agent | Collagenase | Mincing/filtration with buffer solution |
| Incubation for digestion | 45 min at 37°C | 10 min at room temperature |
| Centrifugation for extraction of the pellet | 300 g for 5 min | 2000 g for 10 min |
| Washing of the reagent | Washing with PBS solution | No washing |
| Cell count and viability | ||
| Cell number | 3.38 × 106/cc | 1.34 × 106/cc |
| Cell viability (%) | 82.86% | 85.82% |
| Flow cytometer results | ||
| CD45(−)/CD73(+) CD90(+)/CD73(+) Adipose-derived stem cell content |
20.22% 31.05% |
42.37% 52.08% |
| CD45(−)/CD31(+) Endothelial cell content |
13.60% | 21.06% |
| CD45(+)/CD14(+) Macrophage/monocyte cell content |
23% | 7.28% |
PBS, phosphate-buffered saline.