KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-FLAG (1:2,000)	Sigma-Aldrich	Cat. #F7425; RRID:AB_439687
Rabbit monoclonal anti-IRP2 (1:1,000/1:250)	Cell Signaling Technology	Cat. #37135S; RRID:AB_2799110
Rabbit polyclonal anti-CUL1 (1:1,000)	Thermo Fisher Scientific	Cat. #71–8700; RRID:AB_2534002
Anti-SKP1 (1:5,000)	Michele Pagano’s lab	N/A
Anti-rabbit, HRP-conjugated (1:5,000)	GE Healthcare	Cat. #NA934; RRID:AB_2722659
Mouse monoclonal anti-β-Actin (1:1,000)	Cell Signaling Technology	Cat. #3700S
Mouse monoclonal anti-Flag (1:1,000)	Cell Signaling Technology	Cat. #8146S
Goat anti-Rabbit IgG (H+L) Alexa Fluor Plus 800 (1:10,000)	Invitrogen	Cat. #A32735
Goat anti-Mouse IgG (H+L) Alexa Fluor 680	Invitrogen	Cat. #A28183
Bacterial and Virus Strains
E. coli BL21(DE3)	NEB	Cat. #C2527I
E. coli DH5α	NEB	Cat. #C2987I
E. coli DH10Bac	Life Technologies	Cat. #10361012
Chemicals, Peptides, and Recombinant Proteins
Ammonium ferric citrate (FAC)	Sigma-Aldrich	Cat. #F5879
L-cysteine free base	MP Biomedicals	Cat. #101444
Sodium Dithionite	Acros Organics	Cat. #169590250
Potassium hexacyanoferrate (K3Fe(CN)6)	Sigma-Aldrich	Cat. #31253
Lipofectamine 2000	Invitrogen	Cat. #11668019
Polyethylenimine	Polysciences	Cat. # 24765–1
MG132	Peptides International	Cat. #IZL-3175v
MLN4924	Active Biochem	Cat. # A-1139
TEV protease	In house	N/A
Critical Commercial Assays
SuperSignal West Pico Chemiluminescent Substrate	Thermo Fisher Scientific	Cat. #PI34580
SuperSignal West Femto Chemiluminescent Substrate	Thermo Fisher Scientific	Cat. #PI34095
Deposited Data
IRP2-FBXL5-SKP1 complex model coordinates	This paper	PDB: 6VCD
IRP2-FBXL5-SKP1 complex cryo-EM map	This paper	EMDB: EMD-21149
Experimental Models: Cell Lines
Spodoptera frugiperda: Sf9	Life Technologies	Cat. #B825–01
Trichoplusia ni: HighFive	Life Technologies	Cat. #B85502
Human: HEK293	ATCC	Cat. #CRL-1573
Human: HEK293T	ATCC	Cat. #CRL-3216
Oligonucleotides
Biotinylated Ferritin H IRE RNA	IDT	N/A
Recombinant DNA
pET-FBXL5-SKP1	This paper	N/A
pET-FBXL5 Mutants-SKP1	This paper	N/A
pFB-GST-IRP2	This paper	N/A
pFB-GST-IRP2 Mutants	This paper	N/A
pGEX-CUL1NTD	This paper	N/A
pcDNA5/FRT/TO Empty Vector	Addgene	N/A
pcDNA5/FRT/TO-HA-FLAG-FBXL5C492	This paper	N/A
pcDNA5/FRT/TO-HA-FLAG-FBXL5C492 4C>A	This paper	N/A
pcDNA5/FRT/TO-FLAG-IRP2	Vashisht et al., 2009	https://science.sciencemag.org/content/326/5953/718/tab-figures-data
Software and Algorithms
Phenix	Adams et al., 2012	https://www.phenix-online.org/
Coot	Emsley and Cowtan, 2004	http://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
PyMOL	Pymol	http://pymol.org/2/
UCSF Chimera	Pettersen et al., 2004	https://www.cgl.ucsf.edu/chimera/
Relion-3.0	Zivanov et al., 2018	https://www3.mrc-lmb.cam.ac.uk/relion/
cisTEM	Grant et al., 2018	https://cistem.org
GCTF	Zhang, 2016	https://www.mrc-lmb.cam.ac.uk/kzhang/
MATLLAB 2019a	MathWorks	N/A
EasySpin	Stoll and Schweiger, 2016	http://easyspin.org
Image Studio	LI-COR	https://www.licor.com/bio/image-studio-lite/
Prism 8	GraphPad	N/A
Other
EnSpire reader	PerkinElmer	N/A
Anaerobic glove box	McCoy	N/A
ANTI-FLAG M2 Affinity Gel	Sigma-Aldrich	Cat. #A2220
Glutathione Sepharose 4B	GE healthcare	Cat. #17-0756-05
Ni Sepharose excel	GE healthcare	Cat. #17-3712-02
HiTrap Q-HP, 1mL	GE healthcare	Cat. #17-1153-01
Superdex 200 Increase 10/300 GL	GE healthcare	Cat. #28990944
AlphaScreen Streptavidin-coated donor beads	PerkinElmer	Cat. #6760002S
AlphaLISA anti-GST AlphaScreen acceptor beads	PerkinElmer	Cat. #AL110C
4 mm O.D. quartz tubes	Wilmad-LabGlass	Cat. #707-SQ-250M
EMX X-band spectrometer	Bruker	N/A
ESR900 helium cryostat	OxfordInstruments	N/A