Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Steady-state turnover numbers for different α:β ratios.
| β2 (μM) | α2 (μM) | α2β2a (% β2 in complex) | kcat b (s−1) |
|---|---|---|---|
| 0.1 | 10 | 98 | 9.0 (3) |
| 1 | 10 | 98 | 7.8 (1) |
| 10 | 10 | 96 | 1.45 (4) |
| 1 | 1 | 64 | 4.3 (2) |
| 0.2c | 1 | 81 | 6.8 (3) |
Assuming a Kd for α2β2 of 0.2 μM (ref 13).
Turnover number in the presence of TR/TRR/NADPH. kcat has not been scaled for the percentage of active complex.
These are our standard assay conditions to assess β2 activity.
