Figure 2.

The effect of collisional energy on population of fragment ions derived from dissociation of FGF/pentasaccharide complexes (charge state +7) and their gas-phase mobility represented by (A) two-dimensional IMS-MS plots, (B) extracted CIU profile of the precursor ion and (C-G) conformer-specific fragment ion spectra where the fragment ions are derived from compact, near-native (green), partially unfolded (blue) and fully unfolded (red) precursor ions at different activation energies. The three numbers in parentheses are used to designate composition of GAG fragments (the number of saccharide monomers in the chain, the number of sulfate groups and the number of acetyl groups) following the commonly accepted convention.⁴⁶