Table 2.
Characteristics of the peptides selected for cysteine trioxidation and further MRM analysis.
| SR. No | GS | Peptide sequence with trioxidation site(#)a | Charge state, z | Precursor ion m/z | Precursor ion mass error, ppm | Mascot score | pep_expect |
|---|---|---|---|---|---|---|---|
| 1 | ALB | K.ALVLIAFAQYLQQC#PFEDHVK.L | 2 | 1241.128 | −0.564 | 103.07 | 1.90E-08 |
| 2 | ALB | K.SLHTLFGDKLC#TVATLR.E | 2 | 962.0044 | 0.52 | 114.8 | 8.60E-10 |
| 3 | ALB | K.QEPERNEC#FLQHKDDNPNLPR.L | 3 | 876.4022 | −1.18 | 104.96 | 2.10E-08 |
| 4 | ALB | R.NEC#FLQHKDDNPNLPR.L | 3 | 663.303 | −1.057 | 116.97 | 6.80E-10 |
| 5 | ALB | R.LVRPEVDVMC#TAFHDNEETFLK.K | 3 | 881.0812 | −1.704 | 76.52 | 1.70E-05 |
| 6 | ALB | K.YIC#ENQDSISSK.L | 2 | 717.8068 | 0.279 | 75.01 | 6.10E-06 |
| 7 | ALB | K.LKEC#C#EKPLLEK.S | 2 | 764.8658 | −1.047 | 60.23 | 0.00028 |
| 8 | ALB | K.SHC#IAEVENDEM#PADLPSLAADFVESK.D | 3 | 1005.103 | −5.079 | 67.46 | 6.10E-05 |
| 9 | ALB | K.QNC#ELFEQLGEYKFQNALLVR.Y | 2 | 1295.635 | −0.657 | 120.69 | 4.10E-10 |
| 10 | ALB | K.QNC#ELFEQLGEYK.F | 2 | 824.8621 | −0.971 | 78.46 | 1.10E-05 |
| 11 | ALB | R.RPC#FSALEVDETYVPK.E | 2 | 951.4517 | −0.526 | 92.53 | 2.10E-07 |
| 12 | ALB | K.EFNAETFTFHADIC#TLSEKER.Q | 3 | 846.0486 | 0.592 | 70.59 | 3.90E-05 |
| 13 | ALB | K.ADDKETC#FAEEGKK.L | 3 | 540.2354 | −1.051 | 104.82 | 5.80E-09 |
aThe annotated MS/MS spectra in Mascot Peptide View of the individual peptides are shown in Supplemental Data S1 and S2.