Table 3.
Peptides selected for cysteine carbamidomethylation and further MRM analysis to establish relative retention time.
| SR. No | GS | Peptide sequence with trioxidation site(#) | Charge state, z | Precursor ion m/z | Precursor ion mass error, ppm | Mascot score | pep_expect |
|---|---|---|---|---|---|---|---|
| 1 | ALB | K.ALVLIAFAQYLQQC*PFEDHVK.L | 2 | 1245.647 | 0.964 | 110.83 | 3.10E-09 |
| 2 | ALB | K.SLHTLFGDKLC*TVATLR.E | 2 | 966.5228 | −1.657 | 133.43 | 9.00E-12 |
| 3 | ALB | K.QEPERNEC*FLQHKDDNPNLPR.L | 3 | 879.4144 | −0.645 | 102.54 | 3.40E-08 |
| 4 | ALB | R.NEC*FLQHKDDNPNLPR.L | 3 | 666.3152 | 1.002 | 128.14 | 6.80E-11 |
| 5 | ALB | R.LVRPEVDVMC*TAFHDNEETFLK.K | 3 | 884.0934 | −0.34 | 162.9 | 2.70E-14 |
| 6 | ALB | K.YIC*ENQDSISSK.L | 2 | 722.3252 | 0.277 | 96.98 | 4.50E-08 |
| 7 | ALB | K.LKEC*C*EKPLLEK.S | 2 | 769.3842 | −2.017 | 97.05 | 8.40E-08 |
| 8 | ALB | K.SHC*IAEVENDEM#PADLPSLAADFVESK.D | 3 | 1008.115 | −7.646 | 98.14 | 6.60E-08 |
| 9 | ALB | K.QNC*ELFEQLGEYKFQNALLVR.Y | 2 | 1300.153 | −1.347 | 136.24 | 1.30E-11 |
| 10 | ALB | K.QNC*ELFEQLGEYK.F | 2 | 829.3805 | −0.121 | 113.97 | 3.10E-09 |
| 11 | ALB | R.RPC*FSALEVDETYVPK.E | 2 | 955.97 | −0.052 | 93.48 | 1.60E-07 |
| 12 | ALB | K.EFNAETFTFHADIC*TLSEKER.Q | 3 | 849.0609 | −0.511 | 152.39 | 2.90E-13 |
| 13 | ALB | K.ADDKETC*FAEEGKK.L | 3 | 543.2477 | 1.045 | 130.24 | 2.20E-11 |