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. 2020 Apr 15;3:174. doi: 10.1038/s42003-020-0902-8

Fig. 2. Light masks Sh effects on sleep at high temperature.

Fig. 2

Sleep behaviors in individual male flies were analyzed in constant light (LL) or constant dark (DD) at 21 °C or 29 °C. a, c Sleep profiles of control (gray lines) or Sh mutants (pink lines) during the first two cycles of LL or DD. Data represent average ± SEM (n = 20–48). b, d Box plots represent the total amounts of subjective L or subjective D sleep on the second cycle of LL or DD at 21 °C (blue boxes) or 29 °C (orange boxes) (n = 20–48). Aligned ranks transformation ANOVA detected significant interactions between Sh mutation and temperature on sleep duration in LL (P < 0.0001 for either subjective L or subjective D), but not in DD (P = 0.1002 for subjective L; P = 0.0918 for subjective D). n.s., not significant; ***P < 0.001 as determined by Wilcoxon rank-sum test.