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. 2020 Apr 15;3:174. doi: 10.1038/s42003-020-0902-8

Fig. 4. Ionotropic GABA receptor Rdl in dFSB neurons acts downstream of Sh to suppress sleep.

Fig. 4

23E10-Gal4 driver was genetically combined with Rdl RNAi transgene to deplete endogenous Rdl expression in dFSB neurons of wild-type or Sh mutant flies. Sleep behaviors were analyzed individually in transgenic male flies. Box plots represent the total amounts of L or D sleep on day 2 (21 °C, blue boxes) versus day 5 (29 °C, orange boxes) (n = 20–63). Aligned ranks transformation ANOVA detected significant interactions of Rdl depletion in dFSB neurons with Sh on sleep duration at 21 °C (P < 0.001 for L sleep; P < 0.01 for D sleep), but not at 29 °C. n.s., not significant; *P < 0.05, **P < 0.01, ***P < 0.001 as determined by Wilcoxon signed-rank test.