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. 2020 Apr 15;10:6441. doi: 10.1038/s41598-020-63418-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of Vectashield (VS) on AF(+)647 and AF(+)488 fluorescence intensity. Widefield images of (a) AF(+)647-labelled neurofilament light chain (NfL) in ND7/23 cells, (b) AF(+)488-labelled NfL in ND7/23 cells, (c) AF(+)647-labelled voltage-gated sodium channels (panNav) in mouse cortical neurons (MCN), (d) AF(+)647-labelled ankyrin G (AnkG) in MCN. In panels (a–d), the same field of view is shown for PBS and VS conditions. Brightness and contrast were linearly adjusted to show the same display range in both PBS and VS conditions, as indicated by the look-up table (LUT) intensity scale bars.